Figure 6.

Post-TBI n-3 PUFA treatment enhances oligodendrogenesis. (A) Representative images of 5-bromo-2′-deoxyuridine (BrdU) and adenomatous polyposis coli (APC) immunofluorescence in the ipsilateral perilesion cortex and striatum at 35 days after traumatic brain injury TBI. Boxes indicate areas that were enlarged in high-power images (the sixth column). Arrows: BrdU⁺/APC⁺ cells (yellow). Arrowheads: BrdU⁺/APC⁻ cells (green). Scale bars: 50 μm. (B) Quantification of newly generated mature oligoden-drocytes (BrdU⁺/APC⁺ cells) in the perilesion cortex (CTX) and striatum (STR). ^*p ≤ 0.01, ^***p ≤ 0.001 versus vehicle; ##p ≤ 0.01, ###p ≤ 0.001 versus N3 by one-way analysis of variance (ANOVA). (C, D) Pearson correlation between the mice's spatial learning (C) and memory (D) performance in the Morris water maze and the total number of BrdU⁺/APC⁺ cells in the cortex and striatum. n = 6 mice for sham group; n = 8 mice for vehicle, fatty acid injection (N3), and fish oil dietary supplementation (FO) groups; n = 7 mice for N3 + FO group.