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. Author manuscript; available in PMC: 2017 Sep 27.
Published in final edited form as: Oncogene. 2017 Mar 27;36(30):4299–4310. doi: 10.1038/onc.2017.64

Figure 3. Skp2 deficiency in CRPC cells reduces Twist expression level post-translationally through proteasomal degradation.

Figure 3

(a, b) Twist expression level in PC3 (a) or 22Rv1 (b) cells upon GFP, luciferase, Skp2 or Twist knockdown were analyzed by western blot. Two different Skp2 shRNAs were used in this assay. (c, d) Real-time PCR analysis of Twist in PC3 (c) or 22Rv1 (d) cells with GFP, luciferase, Skp2 or Twist knockdown. Quantified results are presented as means ± SD; *, p < 0.05. (e) Western blot assay for Twist expression level with GFP or Skp2 knockdown in PC3 cells with the absence or presence of proteasome inhibitor, MG132. (f) Western blot assay for Twist expression level in 22Rv1 cells with luciferase or Skp2 knockdown upon treatment of 50 μg/mL of cycloheximide (CHX) at various time points.