Table 2.
Antibodies used in this study
| Primary Antibody | Source | Dilution |
|---|---|---|
| Twist – IP | Santa Cruz | 1:750 |
| Twist – WB | Abcam | 1:1000 |
| Twist – IHC | Biorbyt | 1:100 |
| Flag | Sigma | 1:2000 |
| Skp2 | Invitrogen | 1:2000 |
| Xpress | Invitrogen | 1:10,000 |
| GRP78 | BD Biosciences | 1:10,000 |
| β-actin | Sigma | 1:20,000 |
| N-cadherin | Cell Signaling | 1:3000 |
| E-cadherin-WB | Cell Signaling | 1:3000 |
| E-cadherin-IHC | Cell Signaling | 1:200 |
| Vimentin | Cell Signaling | 1:8000 |
| TCF8/ZEB1 | Cell Signaling | 1:2000 |
| S100A4 | Cell Signaling | 1:500 |
| β-TrCP – PC3 | Genetex | 1:1000 |
| β-TrCP – 22Rv1 | Abcam | 1:1000 |
| α-Tubulin | Sigma | 1:10,000 |
| Lamin B | Santa Cruz | 1:5000 |