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. 2017 Jul 27;7:6743. doi: 10.1038/s41598-017-06331-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SMF effects on intracellular calcium levels and L-type channel subunits. (a) Representative scans from the time-lapse calcium imaging experiments showing some responding cells (white arrows) post KCL stimulation at 30 seconds, (b) Representative calcium response traces, grey arrow represents the KCL stimulation at 30 sec (c) Summary data showing significantly higher calcium transient amplitudes in SMF stimulated OPCs (n = 12 cells), (d) Quantification of mRNA expression levels of CaV1.2 and CaV1.3 in SMF stimulated OPCs as compared to control OPCs. Scale bar = 20 µm. *p < 0.05, **p < 0.01. Error bars indicate SEM.