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. 2017 Jul 27;7:6743. doi: 10.1038/s41598-017-06331-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Investigation of myelination potential of SMF stimulated OPCs in DRG microfluidic chamber. (a) Illustration of microfluidic chamber with axonal and soma compartments connected with 10 μm wide and 500 μm long microchannels. DRG neurons (stained with Calcein) were plated in the somatic chamber and only axons (no cell body) extends to the axonal chamber. (b) Representative image demonstrating the wrapping of axons stained with Neurofilament-200 by MOBP+ oligodendrocytes. 3-D analysis of the z-stack images indicate co-localisation of myelinating cells (green) with axons (red) in the z-domain, (c) Representative image demonstrating an increase in myelinating cells (grey arrow) and decrease in nude axons (yellow arrow) in chambers containing SMF stimulated oligodendrocytes as compared to non-stimulated oligodendrocytes. The control OL chamber has fewer cells making contact with the axons. (d) Quantification of number of MOBP+ oligodendrocytes and nude axons. Scale bar = 20 µm, (**p < 0.01).