Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 27;7:6737. doi: 10.1038/s41598-017-06934-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

TRAF6 overexpression does not inhibit CSFV replication following inhibition of NF-κB activation in PAMs. (a,b) CSFV replication in p65-knockdown CMV-TRAF6 cells. Cells stably expressing CMV or CMV-TRAF6 were transfected with p65-sh2 or shN, followed by CSFV infection. RT-PCR analysed CSFV genome RNA, and viral titres in the supernatant were assessed and expressed as TCID₅₀/ml at 24 and 48 hpi. (c–f) Expression of IFN-β and IL-6 in p65-knockdown CMV-TRAF6 cells. Cells stably expressing CMV or CMV-TRAF6 were transfected with p65-sh2 or shN. Cell lysates and culture supernatant were subjected to RT-PCR and ELISA to determine IFN-β and IL-6 mRNA and protein levels at 24 h, respectively. The data represent the mean ± SD of three independent experiments. **P < 0.01 and ***P < 0.001 calculated using Student’s t-test. ns: not significant.