Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 27;7:6648. doi: 10.1038/s41598-017-07206-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Expression of p27 has no effect on adipocyte differentiation. (A–D) Mouse embryonic fibroblasts (MEFs) prepared from WT or aP2-p27 Tg mice embryos at day 16.5 of gestation were cultured and differentiated into adipocytes by treatment with 0.5 mM IBMX and1 µM Dex for the first two days, and subsequently with 10 µg/ml insulin, 50 nM T3, and 10 µM Tro for six days. Assessment of the expression of aP2 during differentiation (A,C) and cellular lipid staining with Oil Red O at Day 8 (B) showed that there were no differences in adipocyte differentiation between the MEFs of WT and Tg mice. Conversely, the expression of UCP1 mRNA (C) and the number of UCP1-expressing cells (D) were significantly decreased in MEFs from Tg mice, compared to WT mice, and accompanied by increased p27 expression (A) (n = 4 per group, Student’s t-test, *p < 0.05). (E and F) HB2 cells were infected with adenovirus expressing GFP (AD-GFP) or p27 (AD-p27), and were then differentiated by treatment with 10 µg/ml insulin and 50 nM T3 for six days. Cellular lipid staining (E) and quantification of the expression of aP2 and UCP1 (F, n = 4 per group, one-way ANOVA followed by the Tukey–Kramer post-hoc test, *p < 0.05) showed that the forced expression of p27 had no effect on adipocyte differentiation in HB2 cells.