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. 2017 Jul 27;7:6648. doi: 10.1038/s41598-017-07206-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transgenic expression of p27 driven by the aP2 promoter did not affect the generation and function of beige adipocytes. Mice acclimated to a room temperature of 28 °C were injected with saline or CL316,243 (CL; 1 mg/kg/day, s.c.) for 14 days. (A and B) Representative histological images (A) and measurement of the UCP1 content per inguinal-WAT (I-WAT) (B) show that there were no differences between WT and Tg mice with regard to the induction of beige adipocytes by CL. (C–E) Analysis of adipocyte fractions isolated from the I-WAT of WT and Tg mice after CL treatment showed the presence of a similar number of multilocular beige adipocytes (C), similar levels of UCP1 (D), and consumption of a similar amount of oxygen in either the basal or norepinephrine-induced state (E). (F) Chronic CL treatment failed to increase levels of UCP1 per BAT in Tg mice. (n = 6 per group, one-way ANOVA followed by the Tukey–Kramer post-hoc test, different letters indicate significant differences between groups, p < 0.05). (G) Enhancement of oxygen consumption after chronic CL treatment was observed in WT mice but not in Tg mice (n = 6 per group, Student’s t-test, *p < 0.05).