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. 2017 Jul 27;7:6747. doi: 10.1038/s41598-017-06439-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Generation and characterization of AlbT3 transgenic mice. (A) Relative amount of Timp3 mRNA expression in liver, WAT (with adipose tissue), kidney, muscle and heart of wild-type (wt) and AlbT3 mice. (B) TIMP3 and albumin (hepatocyte marker) mRNA expression in purified hepatocytes (Hep) and non-parenchymal cells (NPCs) from AlbT3 and wt mouse livers. Expression of mRNA was determined by qRT-PCR and values normalized to the endogenous control (β-actin). (n = 3 per group, *p < 0.03, **p < 0.01, Student’s t test, data are means ± SEM). (C) Weight and (D) blood glucose concentration. (E) Intraperitoneal glucose tolerance test (IPGTT). (n = 14 per group, data are means ± SEM). a.u.: arbitrary units.