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. 2017 Jul 27;7:6747. doi: 10.1038/s41598-017-06439-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Bile acids (BAs) synthesis and transport regulation in wt and AlbT3 mice after 16 weeks of HFD. (A,B,C) Liver mRNA expression of genes involved in cholesterol export, BAs synthesis and secretion. (normalized to β-actin). (D) Plasmatic and hepatic total BAs levels. (E) mRNA expression of genes related to ileal BAs transporting, (normalized to β-actin). (F) Livers from wt and AlbT3 mice were analyzed for NRG1 in total extract (normalized to Actin), ErbB4 in plasmatic membrane extract (normalized to plasmatic membrane protein loading) and SREBP2 in nuclear extract (normalized to Lamin A/C) by Western blot; a representative cropped image of 3 mice per group is shown. (n = 7 per group; *p ≤ 0.05, **p ≤ 0.02, ***p < 0.01; Student’s t test, data are means ± SEM).