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. 2017 Jul 27;7:6644. doi: 10.1038/s41598-017-06291-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Distal parts of the sinus venosus of Anolis sagrei have the molecular phenotype of a chamber. (A,B) Tbx5 is expressed in the entire sinus venosus myocardium (A), whereas Nkx2.5 is expressed in the distal parts only (B). (C) Expression of Gja5 co-localizes with Nkx2.5. (D) The sinus myocardium is distinct from the atria and ventricle by expressing Tbx18. (E) Overview of Gja5 expression in a near-hatching Anolis sagrei. LSH, left sinus horn; PSH, posterior sinus horn; RSH, right sinus horn; sa, sinuatrial; V, ventricle.