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. 2017 Jul 27;7:6645. doi: 10.1038/s41598-017-06341-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In situ hybridization analysis of miR-15b expression using a digoxigen-labeled locked nucleic acid antisense miR-15b probe in frozen sections of the injured rat brain. miR-15b is highly expressed in many but not all dying, Fluoro-Jade-positive (FJ+) neurons in the hippocampal CA1-3 subfields, in all dying (FJ+) neurons of the dentate gyrus (DG) (A), and in all FJ+ neurons of the cortex (B).