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. 2017 Jul 27;7:6713. doi: 10.1038/s41598-017-07220-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Tetrameric DC-SIGN contributes to more membrane tethers per retraction. (A,B) Averaged FD-curves of CHO cells expressing DC-SIGN-WT, DC-SIGN-N80A, DC-SIGN-ΔCRD, and DC-SIGN-ΔRep (N ≥ 20 cells) were generated by averaging N ≥ 100 FD-curves. For averaging, FD-curves were aligned on the maximum detachment peak (A) or on the 2 nN position (B). (C) The number of membrane tether rupture events observed per retraction of a C. albicans cell being detached from a CHO cell expressing either DC-SIGN-WT, DC-SIGN-N80A, DC-SIGN-ΔCRD, or DC-SIGN-ΔRep (N ≥ 20 cells; N ≥ 100 FD-curves). Data are presented as means ± S.D.