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. 2017 Jul 27;7:6676. doi: 10.1038/s41598-017-06011-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Altered expression of transcriptions factors in Hobit^pos CD56^bright liver NK cells. (a) Histogram showing expression of Blimp-1 (left panel), Eomes (middle panel) and T-bet (right panel) on bulk NK cells in the blood (dark grey) compared to the liver (light grey) and a FMO control (white). (b) Expression (left panel) of Blimp-1 on bulk, CD56^dim and CD56^bright NK cells in the peripheral blood (open symbols) and liver (yellow symbols). Ratio of the expression (right panel) of Blimp-1 in each subset between Liver and Blood. (c) Expression of Eomes on bulk, CD56^dim and CD56^bright NK cells in the blood (open symbols) and liver (yellow symbols). (d) Expression of T-bet on bulk, CD56^dim and CD56^bright NK cells in the blood (open symbols) and liver (yellow symbols). (e) Frequency of Blimp-1^pos, Eomes^pos and T-bet^pos cells among CD56^bright liver NK cells comparing Hobit^pos (black symbol) with Hobit^neg (open symbol). (f) Fold change of the expression (MFI) of Blimp-1, Eomes and T-bet on CD56^bright liver NK cells comparing Hobit^pos with Hobit^neg cells. Wilcoxon matched pair sign rank test was used to determine statistical differences in the scatter plots and Friedman test was used for box plots.