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. 2017 Jul 28;8:152. doi: 10.1038/s41467-017-00218-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Postnatal developmental regulation and functional knockdown of NMDAR-mediated synaptic input onto CGE-derived NGFCs. a, b Confocal images of CA1 regions of hippocampi from Htr3A-EGFP and Htr3A-cre:Ai14 mice illustrating the lamina distribution of CGE-derived INs. Scale bars, 100 μm. c, d Confocal images of biocytin-filled SLM interneuron targeting using the reporter mice with NGFC morphology at P10 and P21 developmental time points. Scale bars, 50 μm. e Schematic depicting the recording configuration and stimulus (stim) employed to evoke glutamatergic synaptic input. f Representative example of a whole-cell voltage-clamp recording from the NGFC illustrated in d illustrating AMPAR and NMDAR-mediated EPSCs in response to SLM afferent stimulation. g Scatterplot of AMPAR- and NMDAR-mediated EPSC peak amplitudes in individual SLM NGFCs targeted using the Htr3A-cre:Ai14 mouse (referred subsequently as simply WT) at two developmental stages P6-11 (gray circles; n = 36) and P16-21 (black circles; n = 31). h NMDAR/AMPAR (NA) peak amplitude ratios in NGFCs at P6-11 (gray circles; n = 36) vs. P16-21 (black circles; n = 31). i NA charge ratio at P6-11 (gray circles; n = 36) vs. P16-21 (black circles; n = 31). j Single trace examples of NMDAR and AMPAR EPSCs in individual NGFCs in WT (black traces) and Htr3A-cre:Ai14:Grin1^flox/flox mouse lines (referred hereafter as Grin1 KO; green traces). Values denote NA peak amplitude ratio in each individual cell. k Cumulative distribution of NA peak amplitude ratios in WT (black lines; n = 31 and 36 for P6-11 and P16-21, respectively) and Grin1 KO (green lines; n = 26 and 46 for P6-11 and P16-21, respectively). Dotted lines in g, h and green shaded box in k denotes NA peak amplitude ratios of 1 or lower. Mann–Whitney U-tests were used for all comparisons (*p < 0.05, **p < 0.01, ***p < 0.001). CGE caudal ganglionic eminence, SLM stratum laconosum moleculare, SO stratum oriens, SP stratum pyramidale, SR stratum radiatum. All n values correspond to the number of cells recorded. Construction of box-whisker plots is detailed in methods