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. 2017 Jul 27;7:6683. doi: 10.1038/s41598-017-05938-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Monocytes populations in peripheral blood. (A) Percentage of the three subsets of monocytes over total monocyte cells: classical (CD14⁺⁺CD16⁻), intermediate (CD14⁺⁺CD16⁺) and non-classical (CD14⁺CD16⁺⁺). (B) Examples of panels from the cytometer (gated for total monocytes population). Values are the mean ± SEM of the following number of individuals: CONTROL n = 28; without MHE (NMHE) n = 30; with MHE (MHE) n = 40. Values significantly different from controls are indicated by asterisks*. Values significantly different in patients with MHE compared to NMHE are indicated by α. (*/α p < 0.05; ***p < 0.001).