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. 2017 Jul 27;7:6674. doi: 10.1038/s41598-017-06136-9

Figure 2.

Figure 2

Dual channel overlay image of the gel-electrophoresis of the PE reaction in the presence of Taq and Vent (exo-) DNA polymerases at 72 °C. PE was performed either with all natural dNTPs for 10 min or using a mixture of dATP, dGTP, dCTP, and Y for varying amounts of time (10, 60, and 120 min). The concentration of the nucleotides was 200 μM (each). Dual-labeled products (red-green bands) with different mobility accumulate in the reaction mixture over the course of the reaction because of Y incorporation. Cy5-labeled strands (red bands) appear over time in the reaction mixture due to modification of the template at the 3′-end. Assignment of bands 3 (M-dT + Y), 4 (M + Y, M-dT + Y + dA, and M-dT + Y + dG), 6 (F), and 7 (F + Y) was made by comparative analysis of electrophoresis and MALDI data.