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. 2017 Jul 27;7:6674. doi: 10.1038/s41598-017-06136-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MS analysis of the PE reaction mixtures with Taq polymerase at 72 °C. PE was performed with a mixture of dATP, dGTP, dCTP, and Y for varying amounts of time (10, 30, and 60 min). The concentration of the nucleotides was 200 μM (each). The peaks were assigned based on their m/z values as follows: (1) M; (2) M + dA; (3) M-dT + Y; (4) M + Y, M-dT + Y + dA and M-dT + Y + dG; (5) M-dT + Y + Y; (6) F; and (7) F + Y. Taq polymerase generates a diverse pool of products because of the enzyme transferase activity and nucleotide exchange via pyrophosphorolysis.