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. 2017 Jul 27;7:6643. doi: 10.1038/s41598-017-06910-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Identification of IGF2R as a target of miR-352 in rat aorta endothelial cells (RAECs). (A) Seed sequence of miR-352, and the complementary 3′-UTR sequence of IGF2R. (B) Luciferase analysis. Luciferase activity of the IGF2R wt 3′-UTR construct but not the IGF2R mut construct, was decreased in the miR-352-expressing cells (*P < 0.05, n = 3). (C–E) Overexpression or inhibition of miR-352 in RAECs resulted in a decrease or an increase in IGF2R expression on both the mRNA (C) and protein (D,E) levels as determined by qPCR (C), western blot (D) and immunofluorescence staining (E), respectively. Specific fluorescence in green, and nuclei staining in blue (***P < 0.001, *P < 0.05, n = 3).