Fig. 2. Effect of alpha-linolenic acid during early- and/ or late-embryogenesis on total cell number of porcine blastocysts at 168 h after in vitro fertiliztion.

(A): fluorescence image of stained nuclear by Hoechst 33342, (B): total cell number of blastocyst. T1: without ALA both early- and late-embryogenesis; T2; with 25 μM ALA at late embryogenesis, T3; with 25 μM ALA at early embryogenesis, T4; with 25 μM ALA for both early- and late-embryogenesis Scale bar: 200 μm. (P<0.05).