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. 2017 Jul 27;7:6684. doi: 10.1038/s41598-017-07096-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TPO induces a time and dose-dependent decrease of TRIB3 mRNA levels. (a) Primary haematopoietic cells were grown under megakaryocyte differentiation conditions with 40 ng/mL TPO for the indicated lengths of time, or (b) for four days with the indicated doses of TPO. Gene expression levels are expressed relative to the respective transcript levels at the initiation of the culture period. Results are representative of three independent experiments. Error bars denote SD of technical replicates (n = 3–6). Error bars smaller than the size of the symbol are not shown.