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. 2017 Jul 27;7:6684. doi: 10.1038/s41598-017-07096-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TRIB3 silencing enhances the megakaryocytic differentiation of UT7/mpl cells. shRNA-transduced cells (shControl and shTRIB3.4) were grown for 7 days in the absence (basal) or presence of 100 ng/mL TPO (+TPO) and then analysed for TRIB3 mRNA and CD61 expression. (a) TRIB3 mRNA levels are expressed relative to the basal levels in shControl. Error bars denote SD of pooled Q-PCR data from two independent cultures (n = 6); (b–d) Flow cytometric analysis of megakaryocytic differentiation. (b) Representative density plots display the percentages of CD61⁺ cells. (c) Representative histogram plots display the median fluorescence intensities (MFI); the faint traces correspond to the fluorescence distribution of samples stained with an isotype-matched control antibody. (d) Results of three independent cultures are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.