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. 2017 Jul 27;7:6684. doi: 10.1038/s41598-017-07096-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TRIB3 silencing enhances the megakaryocytic differentiation of primary haematopoietic cells. shRNA-transduced cells (shControl and shTRIB3.4) were grown for 7 days with 40 ng/mL TPO and then analysed for mRNA and surface marker expression. (a) mRNA levels for the indicated genes are depicted relative to the respective levels in shControl. Error bars denote SD of pooled Q-PCR data from two independent experiments (n = 6). (b,c) Flow cytometric analysis of megakaryocytic differentiation. (b) Representative density plots display the percentages of differentiated cells and histogram plots display the median fluorescence intensities of the cells encompassed within the horizontal markers. (c) Results of four independent experiments are presented as mean ± SD. The top panel shows the percentages of differentiated cells in the transduced cultures and the bottom panel shows the total number of differentiated cells, expressed as a percentage of those in shControl. *P < 0.05 and ***P < 0.001.