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. 2017 Jul 27;7:6684. doi: 10.1038/s41598-017-07096-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TRIB3 silencing enhances the basal levels of ERK1/2 phosphorylation in UT7/mpl cells. shRNA-transduced cells were incubated for 30 min in the absence or presence of 100 ng/mL TPO and then analysed for AKT and ERK1/2 phosphorylation. Phosphoproteins were detected by immunoblotting and quantified by scanning densitometry. A representative immunoblot is shown in (a); quantification is shown in (b). Relative phosphorylation levels were calculated as the ratio of the intensity of the phosphorylated polypeptide bands to the intensity of the corresponding total polypeptide bands. Results are expressed relative to the level of phosphorylation measured in unstimulated shControl cells. Error bars denote SD of three independent cultures. *P < 0.05.