Figure 5.

Normalized synergy correlates with functional output and peptide sensitivity. (A) Traditional synergy versus maximum CD107a expression, defined in Figure 3. (B) Normalized synergy versus maximum CD107a expression, defined in Figure 3. (C) Traditional synergy versus peptide sensitivity, defined in Figure 3. (D) Normalized synergy versus peptide sensitivity, defined in Figure 3. (E) Expression of CD107a on populations of functional HCV-specific CTLs after phorbol 12-myristate 13-acetate and ionomycin stimulation with respect to two-dimensional (2D) T cell receptor (TCR) affinity as measured by micropipette adhesion frequency assay. All data points for CD107a expression were shown as mean ± SD calculated from duplicates. The same results were obtained for the two peptide sensitivity experiments performed. Therefore, no error bar was shown in (C,D). ρ and p values determined by Spearman’s rank correlation.