Figure 6.

CD8-independent T cell receptor (TCR) is able to retain functional response in the presence of CD8 blocking antibody. (A) Percent of CD8 co-receptor that was blocked for each concentration of CD8 blocking antibody present during the 4 h of HCV peptide pulsed JY cell stimulation (Figure S6 in Supplementary Material). (B) Percentage of CD107a positive T cells stimulated with varying concentrations of CD8 blocking antibody for CD8-independent TCR (TCR10) and CD8-dependent TCR (TCR5). 10 µM of HCV peptide was presented throughout the stimulation. Percentage of CD107a positive T cells for each CD8 blocking condition was normalized to normal stimulation condition without CD8 blocking. Data in panel (A,B) were mean ± SD averaged from two independent experiments from two different days. One-way ANOVA was performed between normalized CD107a expression to assess statistical significance between values with p-value denoted above data.