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. 2017 Jul 28;10:234. doi: 10.3389/fnmol.2017.00234

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Copyright © 2017 Pastuzyn and Shepherd.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Arc and Ube3A do not interact in the hippocampus in vivo. (A) Arc was immunoprecipitated from whole hippocampal lysate from P30 WT mice and immunoblotted for Arc and Ube3A. Arc and Ube3A do not coimmunoprecipitate. (B) Hippocampal lysates from P30 WT mice underwent subcellular fractionation and Arc was immunoprecipitated from the nuclear and synaptoneurosome fractions. Arc and Ube3A do not coimmunoprecipitate. I, input; IB, immunoblot; nuc, nucleus; synapto, synaptoneurosome; U, unbound fraction.