Table 2.
Cardiac mitochondrial proteins that are hypoacetylated at lysine residues in diabetic+methylene blue (DB+MB) versus diabetic (DB) group
| Protein name and abbreviation/peptide sequence | Swiss-Prot accn# | Lysine position | Acetylation ratio |
|---|---|---|---|
| Electron transport chain | |||
| ATP synthase subunit alpha (ATP5a1) | P15999 | ||
| K.GPVGSKIR.R | 167 | 0.50 | |
| R.FNDGTDEKKK.L | 239 | 1.02 | |
| R.SDGKISEQSDAK.L | 531 | 0.24 | |
| ATP synthase subunit beta (ATP5b) | P10719 | ||
| K.VLDSGAPIKIPVGPETLGR.I | 133 | 0.35 | |
| ATP synthase subunit b (AT5f1) | P19511 | ||
| K.AQQALVQKR.H | 162 | 0.38 | |
| R.EKAQQALVQK.R | 154 | 0.28 | |
| ATP synthase subunit gamma (ATPg) | P35435 | ||
| K.IKSILYR.T | 113 | 0.34 | |
| K.SIKNIQK.I | 14 | 0.40 | |
| R.THSDQFLVSFKDVGR.K | 129 | 0.40 | |
| ATP synthase subunit d (ATP5h) | P31399 | ||
| K.YNALKIPVPEDK.Y | 78 | 0.16 | |
| R.ANVDKPGLVDDFK.N | 63 | 0.34 | |
| R.ANVDKPGLVDDFKNK.Y | 71 | 0.26 | |
| ATP synthase subunit O (ATPo) | Q06647 | ||
| K.RLDQVEKELLR.V | 60 | 0.33 | |
| R.LDQVEKELLR.V | 60 | 0.35 | |
| ADP–ATP translocase 1 (ANT1) | Q05962 | ||
| K.DFLAGGIAAAVSKTAVAPIER.V | 23 | 0.37 | |
| K.IFKSDGLK.G | 166 | 0.22 | |
| R.YFPTQALNFAFKDK.Y | 92 | 0.79 | |
| ADP–ATP translocase 2 (ANT2) | Q09073 | ||
| R.YFPTQALNFAFKDK.Y | 92 | 0.79 | |
| Cytochrome b-c1 complex subunit 1 QCR2c1 | Q68FY0 | ||
| K.ALSKDLPK.V | 38 | 1.51 | |
| Cytochrome b-c1 complex subunit 2 (QCR2c2) | P32551 | ||
| K.GASSFKITR.G | 97 | 0.23 | |
| R.GGLGLAGAKAK.Y | 149 | 0.34 | |
| R.LASTLTTKGASSFK.I | 91 | 0.20 | |
| R.SQLKIDK.A | 159 | 0.39 | |
| Cytochrome c oxidase subunit 4 isoform 1 (COX41) | P10888 | ||
| K.LLSASQKALK.E | 60 | 0.25 | |
| Glucose oxidation | |||
| Dihydrolipoyl dehydrogenase (DLD) | Q6P6R2 | ||
| K.ALTGGIAHLFKQNK.V | 143 | 0.21 | |
| K.ILGHKSTDR.I | 445 | 0.23 | |
| K.SEEQLKEEGVEFK.V | 409 | 0.15 | |
| R.ILQKQGFK.F | 267 | 0.20 | |
| R.RPFTQNLGLEELGIELDPKGR.I | 334 | 0.29 | |
| R.SAVKALTGGIAHLFK.Q | 132 | 0.06 | |
| Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex (ODO2) | Q01205 | ||
| R.DAFIKKN KDAFLKKH | 273 | 0.23 | |
| R.HKDAFLK.K | 268 | 0.30 | |
| R.HKDAFLKK.H | 268, 273 | 0.05 | |
| FA oxidation | |||
| 2,4-dienoyl-CoA reductase (DECR) | Q64591 | ||
| K.QLIKAQK.G | 185 | 0.20 | |
| Acetyl-CoA acetyltransferase (ACAT1) | P17764 | ||
| K.VLKYAGLK.K | 335 | 0.21 | |
| K.YAGLKK.E | 340 | 0.24 | |
| R.GATPYGGVKLEDLIVK.D | 171 | 0.16 | |
| Acyl-coenzyme A thioesterase 1 (ACOT1) | O88267 | ||
| R.DEKGALFR.A | 42 | 0.20 | |
| K.VPASKAFTGFIVEADTPGIHIGK.K | 217 | 0.87 | |
| Acyl-coenzyme A thioesterase 2 (ACOT2) | O55171 | ||
| R.DEKGALFR.A | 83 | 0.20 | |
| R.DVQKPYVVELEVLDGHEPDGGQR.L | 141 | 0.34 | |
| Carnitine O-acetyltransferase (CRAT) | Q704S8 | ||
| R.VPGLKQDSVVNFLK.S | 186 | 0.12 | |
| Enoyl-CoA delta isomerase 1 (ECI1) | P23965 | ||
| K.LENDKSIR.G | 76 | 0.23 | |
| Hydroxyacyl-coenzyme A dehydrogenase (HADH) | Q9WVK7 | ||
| K.TLSSLSTSTDAASVVHSTDLVVEAIVENLKLK.N | 125 | 0.91 | |
| Long-chain specific acyl-CoA dehydrogenase (LCAD) | P15650 | ||
| K.AFGKTVAHIQTVQHK.L | 322 | 0.33 | |
| Medium-chain specific acyl-CoA dehydrogenase (MCAD) | P08503 | ||
| K.VPASKAFTGFIVEADTPGIHIGK.K | 217 | 0.87 | |
| Short-chain specific acyl-CoA dehydrogenase (SCAD) | P15651 | ||
| R.HAFGAPLTKLQNIQFK.L | 306 | 0.31 | |
| Trifunctional enzyme subunit alpha (HADHa) | Q64428 | ||
| K.DTTASAVAVGLKQGK.V | 531 | 0.21 | |
| K.TSKDTTASAVAVGLK.Q | 519 | 0.14 | |
| R.DSIFSNLIGQLDYKGFEK.A | 436 | 0.47 | |
| R.FVDLYGAQKVVDR.L | 728 | 0.29 | |
| R.ILQEGVDPKK.L | 569 | 0.15 | |
| R.INSPNSKVNTLNK.E | 60 | 0.34 | |
| Trifunctional enzyme subunit beta (HADHb) | Q60587 | ||
| R.TNIPKDVVDYIIFGTVIQEVK.T | 96 | 0.04 | |
| TCA cycle | |||
| Aconitate hydratase (ACO2) | Q9ER34 | ||
| K.FNPETDFLTGKDGK.K | 517 | 0.22 | |
| K.IHPVDKLTIQGLK.D | 723 | 0.22 | |
| R.AIITKSFAR.I | 689 | 0.36 | |
| R.YDLLEKNINIVR.K | 50 | 0.38 | |
| Aspartate aminotransferase (GOT2) | P00507 | ||
| K.AEAQIAGKNLDK.E | 90 | 0.21 | |
| R.FFKFSR.D | 150 | 0.30 | |
| R.KAEAQIAGK.N | 82 | 0.22 | |
| R.KAEAQIAGKNLDK.E | 90 | 0.14 | |
| R.TQLVSNLKK.E | 363 | 0.23 | |
| Fumarate hydratase | P14408 | ||
| K.KPVHPNDHVNK.S | 170 | 0.28 | |
| Isocitrate dehydrogenase [NADP] (IDH2) | P56574 | ||
| K.DIFQEIFDKHYK.T | 272 | 0.24 | |
| K.HYKTDFDK.N | 275 | 0.23 | |
| K.LILPHVDVQLKYFDLGLPNR.D | 80 | 0.33 | |
| K.LVFTPKDGSGAK.E | 199 | 0.28 | |
| K.TDFDKNK.I | 280 | 0.20 | |
| R.DQTNDQVTIDSALATQKYSVAVK.C | 106 | 0.48 | |
| R.FKDIFQEIFDKHYK.T | 269 | 0.18 | |
| R.GKLDGNQDLIR.F | 384 | 0.28 | |
| R.HAHGDQYKATDFVVDR.A | 180 | 0.26 | |
| Malate dehydrogenase (MDH2) | P04636 | ||
| K.GLEKNLGIGK.I | 301 | 0.28 | |
| K.KGEDFVK.N | 329 | 0.19 | |
| K.KGLEKNLGIGK.I | 301 | 0.34 | |
| K.KHGVYNPNK.I | 157 | 0.28 | |
| K.NLGIGKITPFEEK.M | 307 | 0.25 | |
| R.ANTFVAELKGLDPAR.V | 185 | 0.23 | |
| Other | |||
| ES1 protein homolog | P56571 | ||
| K.GVTEAHVDQKNK.V | 131 | 0.33 | |
| NEDD8-conjugating enzyme (UBE2f) | Q5U203 | ||
| R.VSVRDKLLVK.E | 35, 39 | 0.29 | |
| Phenylalanine–tRNA ligase alpha subunit (FARSa) | Q505J8 | ||
| R.VDKSAADGPR.V | 117 | 0.88 | |
| Stress-70 protein (HSPa9a) | P48721 | ||
| R.AQFEGIVTDLIKR.T | 348 | 0.64 | |
| R.HIVKEFK.R | 288 | 0.20 | |
Bold and underlined acetylated lysine residue in analyzed peptide fragment
Lysine position given refers to full protein sequence
Acetylation ratio reported as DB−MB:DB