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. 2017 Jul 18;5(14):e13307. doi: 10.14814/phy2.13307

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© 2017 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Chronic contractile activity (CCA) induces mitochondrial adaptations in skeletal muscle by day 7. Fold‐change in the protein expression of mitochondrial biogenesis markers (A) PGC‐1α, (B) COX I, and (C) COX IV (n = 8 per group). (D) Fold‐change in the COX activity for mitochondrial contents (n = 6 per group). (E) Representative blots for PGC‐1α, COX I, and COX IV. β‐Actin was used as loading control. Molecular weights on the left of blots are for protein ladders. All bars are indicative of fold‐change relative to the control group at Day 1, and they represent means ± SEM. †P < 0.05, interaction effect between CCA and time; §P < 0.05, main effect of CCA; ¶P < 0.05, main effect of time; *P < 0.05, significant difference versus control at Day 1.