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. 2017 Jul 19;15(7):231. doi: 10.3390/md15070231

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The cytograms of mixotrophic C. zofingiensis during cultivation based on FCM analysis. (a) FSC vs. FL1; (b) Cell count vs. FL1 (533 nm ± 15 nm); (c) FSC vs. FL2; and (d) Cell count vs. FL2 (585 nm ± 20 nm). Green dotted lines (or dots) represent the initial cells (the start of culture); orange solid lines (or dots) represent the final cells (the end of culture). M1 represents the population of small cells with low fluorescence; M2 corresponds to the population of large cells with high fluorescence.

The cytograms of mixotrophic C. zofingiensis during cultivation based on FCM analysis. (a) FSC vs. FL1; (b) Cell count vs. FL1 (533 nm ± 15 nm); (c) FSC vs. FL2; and (d) Cell count vs. FL2 (585 nm ± 20 nm). Green dotted lines (or dots) represent the initial cells (the start of culture); orange solid lines (or dots) represent the final cells (the end of culture). M1 represents the population of small cells with low fluorescence; M2 corresponds to the population of large cells with high fluorescence.