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. Author manuscript; available in PMC: 2017 Jul 28.
Published in final edited form as: Biochem Biophys Res Commun. 2016 May 10;475(1):100–106. doi: 10.1016/j.bbrc.2016.05.046

Fig. 3. Ssa1p was preferentially associated with the rapidly degraded Ub-P-gal.

Fig. 3

Wild-type cells expressing M-gal or Ub-P-gal were grown at 28°C in the presence of 2% galactose for 12–16 h. 3–4 mg of cell proteins were prepared and applied to a 1-ml column of p-aminobenzyl-1-thio-β-galactoside crosslinked to agarose beads at 4°C. The bound β-gal were eluted with lactose and the presence of Ssa1p or β-gal fusion proteins was determined by Western-blot with anti-human Hsp70 or anti-β-gal antibodies and also the β-gal activity was also measured.