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pH and pD profiles of steady-state activity of MhN2OR at 297 K in μmol N2O min−1 mg−1, fit with a speciation curve generated by pKa(H) values of 6.19 ± 0.05 and 9.15 ± 0.05 (blue) and pKa(D) values of 6.4 ± 0.1 and 9.5 ± 0.1, with a solvent deuterium isotope effect of 1.12 ± 0.06 (red).
