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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Ann Surg Oncol. 2016 Jul 21;23(11):3459–3466. doi: 10.1245/s10434-016-5432-8

Table 1.

Checklist of key steps for the sentinel lymph node biopsy procedure in breast cancer.

Consider SLNB for all invasive breast cancer and cases of DCIS undergoing mastectomy
Utilize dual tracer (blue dye and radiocolloid) to optimize identification and reduce false negative rates, especially following neoadjuvant therapy, prior breast/axillary surgery or in patients with elevated BMI
Consider IV prophylaxis if blue dye utilized
Inject blue dye around tumor periphery, at the palpable edge of the biopsy cavity, or into the subareolar plexus
Inject radiocolloid peritumorally, intradermally, or into the subareolar plexus
Avoid injection into the tumor itself or into a seroma cavity
Consider lower dose or subareolar injection for tumors located in the axillary tail
Massage breast can be performed
Remove any suspicious palpable nodes

Abbreviations: SLNB=sentinel lymph node biopsy; DCIS=ductal carcinoma in situ; BMI=body mass index; IV=intravenous