Fig. 3.

Administration of CSCs promotes myocardial regeneration. (A) Large transverse section halfway between the base and the apex of the left ventricle, showing an infarct in a CSC-treated rat. (Inset) Regenerated infarcted myocardium (EGFP and α-sarcomeric actin, yellow-green). (Bars, 1 mm and 100 μm.) (B) Another example of regenerated infarcted myocardium (arrows) is shown first by α-sarcomeric actin staining (red), then by EGFP labeling (green) and then by the combination of EGFP and α-sarcomeric actin (yellow-green). (C) EGFP^POS (green), α-sarcomeric actin^POS (red), small newly formed myocytes within the infarcted region express in their nuclei GATA-4 (white) and MEF2C (magenta). (D) EGFP^POS (green), cardiac myosin heavy chain^POS (red) small newly formed myocytes within the infarcted region express in their plasma membrane connexin 43 (magenta, arrowheads). (E) Newly formed myocytes in the surviving noninfarcted LV myocardium (arrows) are shown first by EGFP labeling (green) and then by the combination of EGFP and α-sarcomeric actin (yellow-green) [Bar (B–E), 10 μm.]