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. 2017 Apr 1;6(4):e004820. doi: 10.1161/JAHA.116.004820

Figure 2.

Figure 2

Bilirubin inhibits the migration of THP‐1 monocytes across activated HUVEC monolayers. A, Time course for migration of CellTrace Far Red–labeled THP‐1 cells across confluent HUVEC monolayers that were preincubated with (squares) or without (circles) 5 ng/mL of TNF‐α for 24 hours. Studies were conducted in the presence of 20 μmol/L of bilirubin (BR; white symbols) or the bilirubin vehicle (Veh; black symbols). Displayed is the percentage of THP‐1 cells in the lower chamber of the transwell (expressed relative to the total number added to the upper chamber) at the indicated time points. B, Dose‐dependent effect of 10 (BR‐10) and 20 (BR‐20) μmol/L of bilirubin on THP‐1 migration at 120 minutes expressed as the percentage of TNF‐stimulated migration in the presence of the bilirubin vehicle. Bars reflect the mean (±SEM) of 4 sets of experiments. *P<0.05 vs No TNF, TNF+BR; **P<0.05. HUVEC indicates human umbilical vein endothelial cell; TNF‐α, tumor necrosis factor alpha.