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. 2017 Apr 1;6(4):e005146. doi: 10.1161/JAHA.116.005146

Figure 5.

Figure 5

Effect of p53 and p21 silencing in EPCs. A, Silencing of p53 gene in mouse EPCs (mEPCs) by lentivirus as a vector demonstrates improved survival in hyperglycemic condition compared to null lenti (non‐silenced) transduced cells. Numbers of viable mEPCs are shown at days 1, 3, and 7 postexposures to high glucose (20 mmol/L). B, Gene expression analysis showed, p53 silencing in mouse EPCs by adenovirus down regulated p53 and p21 gene. Although, p53 silencing did not impair mRNA expression levels of critical endothelial marker gene such as endothelial nitric oxide synthase (eNOS), von Willebrand factor (vWF), and platelet endothelial cell adhesion molecule 1 (PECAM1). C, Human EPCs (CD34+) were transduced to silence p53 and p21 genes using adenovirus constructs and then exposed to high glucose for 48 hours. Silencing of both p53 and p21 in hEPCs were confirmed by the reduced gene expression in comparison to Ad‐null transduced cells, exposed to hyperglycemia. *P<0.05.