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. 2017 Apr 3;6(4):e005330. doi: 10.1161/JAHA.116.005330

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© 2017 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Vinculin (VCL) interacts with cardiac sodium channel (SCN5A) in vivo and in vitro. A, Heart tissue (HT) lysates from mouse were immunoprecipitated (IP) using SCN5A or VCL antibody and analyzed by western blotting using the indicated antibodies. Because of the negative expression of SCN5A, mouse liver tissue was used as a control. (B) SCN5A and wild‐type VCL (VCL‐WT) were transfected into HEK293 cells for 1 day, and cell lysates were IP and analyzed by western blotting. (C) VCL‐D841H and SCN5A were transfected into HEK293 cells for 1 day, and cell lysates were IP and analyzed by western blotting. (D) VCL‐WT, VCL‐D841H, and SCN5A were transfected into HEK293 cells for 1 day, and cell lysates were IP and analyzed by western blotting. IgG indicates immunoblobulin G; MW, molecular weight.