Table 4.
Biophysical Properties of Sodium Channels in HEK293 Cells Coexpressing SCN5A and Either VCL‐WT or ‐D841H
| Samples | Peak I Na | Activation | Inactivation | Late I Na | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| pA/pF | n | V1/2 (mV) | k | n | V1/2 (mV) | k | n | % | n | |
| WT at pH 7.4 | −145±16 | 20 | −41.9±0.8 | 5.0 | 20 | −84.9±1.4 | 5.1 | 26 | 0.31±0.06 | 16 |
| D841H at pH 7.4 | −103±12a | 29 | −41.0±0.7 | 5.0 | 26 | −85.5±1.1 | 5.0 | 29 | 0.32±0.05 | 16 |
| WT at pH 7.0 | −100±12a | 24 | −43.8±0.8 | 5.5 | 18 | −87.1±0.9 | 5.0 | 20 | 0.45±0.23 | 7 |
| D841H at pH 7.0 | −83±16a | 18 | −44.9±1.5 | 5.4 | 18 | −87.9±0.6a | 5.0 | 15 | 0.39±0.19 | 5 |
Values are mean±SE for n experiments. The late I Na level was described as a percentage of peak I Na. All parameters were analyzed using 1‐way ANOVA followed by a Bonferroni test. I Na indicates sodium current; k, slope factor; pA/pF, current density; V1/2, voltage of half‐maximal activation/inactivation; VCL, vinculin; WT, wild type.
a
P<0.05 vs WT at pH 7.4.