Table 7.
Effect of Polymorphisms of SCN5A and VCL on Late Sodium Current in HEK293 Cells
| Samples | Peak I Na | Activation | Inactivation | Late I Na | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| pA/pF | n | V1/2 (mV) | k | n | V1/2 (mV) | k | n | % | n | |
| SCN5A‐WT+VCL‐WT | −101±11 | 17 | −39.4±0.7 | 5.0 | 24 | −84.4±0.9 | 5.0 | 22 | 0.32±0.04 | 29 |
| SCN5A‐WT+VCL‐D841H | −70±9a | 24 | −38.2±0.6 | 5.0 | 26 | −84.8±0.8 | 5.0 | 26 | 0.34±0.05 | 15 |
| SCN5A‐H558R+VCL‐WT | −105±9 | 25 | −38.5±1.1 | 5.0 | 32 | −85.4±1.2 | 5.0 | 30 | 0.43±0.05 | 19 |
| SCN5A‐H558R+VCL‐D841H | −102±7 | 29 | −39.4±0.5 | 5.0 | 38 | −86.0±0.8 | 5.0 | 31 | 0.60±0.06a | 23 |
Values are mean±SE for n experiments. The late I Na level was described as a percentage of peak I Na. All parameters were analyzed using 1‐way ANOVA followed by a Bonferroni test. I Na indicates sodium current; k, slope factor; pA/pF, current density; SCN5A indicates cardiac sodium channel; V1/2, voltage of half‐maximal activation/inactivation; VCL, vinculin; WT, wild type.
a
P<0.05 vs SCN5A‐WT+VCL‐WT.