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. 2017 Apr 24;6(4):e005364. doi: 10.1161/JAHA.116.005364

Figure 4.

Figure 4

MiR‐204 modulates the expression of both runt‐related transcription factor 2 (Runx2) and osterix (Osx) in human aortic valve interstitial cells (AVICs). A, Representative immunoblots and quantitative analysis show that transfection of AVICs of calcified valves with miR‐204 mimic markedly reduces the levels of Runx2 and Osx examined at 72 hours after transfection. B, MiR‐204 antagomir upregulates the levels of Runx2 and Osx at 72 hours after transfection in AVICs of normal valves. Data are expressed as mean±SE. n=8 separate experiments using distinct cell isolates; ANOVA and nonparametric Kruskal–Wallis H test; *P<0.05 vs untreated control; † P<0.05 vs miR‐C control. C, Normal AVICs were infected with lentiviral luciferase reporter of Runx2 3′ untranslated region (UTR) or Osx 3′ UTR together with irrelevant oligonucleotide (miR‐C), miR‐204 mimic, or miR‐204 antagomir. Luciferase assays were performed 72 hours after infection. Quantitative analysis of 3′ UTR luciferase activities. Activities of Runx2 and Osx 3′ UTR reporters are decreased by miR‐204 mimic and increased by miR‐204 antagomir. Data are expressed as median and 95% CI (Runx2 and Osx reporter activity data). n=8 separate experiments using distinct cell isolates; ANOVA and nonparametric Kruskal–Wallis H test; *P<0.05 vs control (miR‐C).