Table 3. Purification and characterization of NUDEL-oligopeptidase from rat brain cytosol.
| Activity, %
|
Total activity, milliunits
|
Protein, mg
|
NUDEL-oligopeptidase, μg
|
Specific activity, milliunits/μg
|
||
|---|---|---|---|---|---|---|
| Enzyme | -NOAB | +NOAB | ||||
| Cytosol plus MO inhibitor | 100 | 17 | 4.55 | 3.8 | 0.167 | 27.25 |
| Pool I | 2.2 | 2.4 | 0.42 | 0.7 | — | — |
| Pool II | 6.1 | 5.8 | 0.85 | 1.4 | — | — |
| Pool III | 4.1 | 4.2 | 0.46 | 0.9 | — | — |
| Pool IV | 3.8 | 2.3 | 0.21 | 0.7 | — | — |
| Pool V | 81.0 | 0.0 | 2.14 | 0.4 | 0.003 | 713.4 |
The protein concentration, the oligopeptidase activities of Pools I–V obtained from the experiment described in Fig. 1, and the susceptibilities of each pool to inhibition by the NOAB inhibitor are described in Materials and Methods. —, not determined.