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. Author manuscript; available in PMC: 2017 Jul 28.
Published in final edited form as: Circ Res. 2015 Oct 15;118(1):20–28. doi: 10.1161/CIRCRESAHA.115.307697

Figure 7. A, Fluorescence microscopy of heart sections from TnT-Cre;CyclinA2-LacZ-enhanced green fluorescent protein (EGFP) mice at PN14 and PN15 stained for CyclinA2-EGFP, Troponin T, 5-ethynyl-2′-deoxyuridine (EdU), and DAPI.

Figure 7

Mice were labeled with EdU during indicated time frames. CyclinA2-EGFP-positive cells were extremely rare. A single CyclinA2-EGFP-positive cardiac myocyte in the field is shown (white circle). CyclinA2-EGFP-positive but EdU-negative cardiac myocytes were also observed. Scale bar: 50 μm. B, Fluorescence microscopy of heart sections from TnT-Cre;CyclinA2-LacZ-EGFP mice at PN14 stained for CyclinA2-EGFP, PDGFRα, CD31, CD45, CD146, EdU, and DAPI. All EdU-positive/ CyclinA2-EGFP-negative cells were labeled with either anti-PDGFRα, anti-CD31, anti-CD45, or anti-CD146 antibody (blue circles). Scale bar: 50 μm. C, Quantitative analysis of CyclinA2-EGFP myocardial cells throughout PN14 and PN15. Note gradual and steady decrease.