Table 3.
Bacterial strains and plasmids used or created in this study.
| Strain or plasmid | Genotype or description | Source or reference |
| Strains | ||
| Escherichia coli | ||
| XL1-Blue | K12 supE44 hsdR17 recA1 endA1 gyrA46 thi | Laboratory collection |
| relA1 lac- F' [proAB+lacIqlacZ Δ M15 Tn10(tetR)] | ||
| Bacillus subtilis | ||
| 168 | trpC2 | [36] |
| BSIP7004 | trpC2 speD::spc | [2] |
| BFS1850 | trpC2mtnK::lacZ | This worka |
| BFS1851 | trpC2mtnU::lacZ | This worka |
| BSHP1850 | trpC2speD::spc mtnT::lacZ | This work |
| BSHP7010 | trpC2 mtnS::spc | This work |
| BSHP7035 | trpC2pmtnK::Tn10 | This work |
| Plasmids | ||
| pUC19 | cloning vector, AmpR | [37] |
| pIC333 | mini-Tn10 delivery vector, SpcR, EryR | [32] |
| pMutin4mcs | cloning vector, ErmR | [31] |
| pDU1850 | pMutin4mcs::mtnK | This work |
| pDU1851 | pMutin4mcs::mtnU | This work |
| pHPP7010 | pUC19mtnS::spcb | This work |
a. This strain has been constructed during the frame of the EC project for the functional characterization of the genome of B. subtilis in Europe b.spc is the spectinomycin resistance gene from Staphylococcus aureus