Fig. 1.

—Entylia carinata (Membracidae) host and fluorescence in situ hybridization of bacteriome organs containing the bacterial symbionts, “Ca. Sulcia muelleri” (red; Bacteroidetes) and “Ca. Nasuia koganicola” (green; Betaproteobacteria). DNA is counterstained with DAPI (blue) primarily showing host DNA. (A) Image showing E. carinata adult. (B) Lateral habitus of E. carinata nymph abdomen and (C) showing bacteriocytes containing bacteria (white arrows illustrate Nasuia containing bacteriocytes). White scale bars represent 200 µm (B) and 100 µm (C). Note: Green autofluorescence observed in abdominal cavity (without arrows) for Image (C). Photograph of E. carinata adult (A) provided by Kyle Kittelberger.