Fig. 2.

—The relationship between percent G + C nucleotide content and genome size of the major co-primary bacterial symbiont lineages in the Auchenorrhyncha (Hemiptera). Symbionts are color-coded by their lineage of origin (discussed in text; see labels, e.g., Hodgkinia = grey). Inset graph shows that G + C content is significantly correlated with genome size (Phylogenetic Generalized Lease Squares [PGLS]: correlation = −0.98 and P-value = <0.001) in sequenced Sulcia lineages. Sulcia phylogeny reconstructed with 16S rRNA using ML criteria in RAxML (Stamatakis 2014) and PGLS performed in R with nlme and ape (Paradis et al. 2004; Pinheiro et al. 2017). Representative host insects are abbreviated as follows: ALF = Macrosteles quadrilineatus, PUNC = M. quadripunctulatus, and ENCA = Entylia carinata.