Figure 6.

Cancer-patient-derived MV induce a tumour-supporting phenotype in human macrophages. (a) Human macrophages were stimulated with MV isolated from cancer or control patients (50 µg/ml) for 24 h and gene expression of VEGFA, WNT5A and IL1B was measured by qRT-PCR (n = 4, *p = 0.004, **p = 0.041 with two-sided t test). Expression changes were calculated relative to unstimulated macrophages. Box plots illustrate the 25–75 percentiles (boxes), the 10–90 percentiles (whiskers) and the median (line). (b) Representative western blot of four independent experiments: Wnt5a expression in unstimulated macrophages (ctl) and after incubation with MV (50 µg/ml, 24 h) from cancer and control patients. Hsp90 and Ponceau staining are shown as loading controls.