Table 1.

Instrument settings for “identical settings” setup.

	“Identical settings” setup	Instruments 1 and 2
	Sample/vesicle type	Sample dilution	Camera level	Detection threshold
EVs	Exosomes from PC-3 cells	200	12	3
	Exosomes from Jurkat cells	1000	13	4
	OMV from Neisseria meningitidis	10,000	12	3–4
	Microvesicles from monocytes	400	12	3–4
Artificial vesicles/beads	Artificial vesicles (Invivofectamine® 2.0)	100,000	13–14	3–4
	Polystyrene latex beads 100 nm	500	8	4
	Silica microspheres 150 nm	100,000	10	4

Samples were analysed using NTA 2.3 build 17 software (Malvern).