Table 3.
Intra-assay variation (within day, n = 6) of size and concentration measurements on instrument 1, using identical settings in NTA 2.3 build 17 software.
| Intra-assay (within day) | Mean size |
Mode size |
Mean concentration |
||||
|---|---|---|---|---|---|---|---|
| Sample/vesicle type | (nm) | % CV | (nm) | % CV | E8 particles/mL | % CV | |
| EVs | Exosomes from PC-3 cells | 128 | 2 | 94 | 5 | 5.8 | 5 |
| OMV from Neisseria meningitidis | 138 | 2 | 104 | 4 | 6.4 | 5 | |
| Microvesicles from monocytes | 141 | 1 | 103 | 4 | 6.8 | 7 | |
| Artificial vesicles/beads | Artificial vesicles (Invivofectamine® 2.0) | 87 | 2 | 81 | 4 | 5.4 | 12 |
| Polystyrene latex beads 100 nm | 102 | 2 | 94 | 3 | 4.9 | 8 | |
| Silica microspheres 150 nm | 140 | 2 | 132 | 2 | 7.8 | 8 | |
| Overall mean % CV | 2 | 3 | 8 | ||||
| Range % CV | 1–2 | 2–5 | 5–12 | ||||