Figure 3.

Western blot analysis of frontal cortex and associated vesicles. Equivalent protein from human frontal cortex brain homogenate without (BH) and with collagenase (BH + C) and three vesicle fractions were separated by gel electrophoresis and the total protein load was visualized using stain-free technology or ponceau S membrane staining. Immunoblotting was carried out using antibodies to BiP, calnexin, calreticulin, VDAC,, flotillin-1, syntenin, TSG101 and CD81. Immunoblots are representative of at least five independent experiments.