Table 1. Positions of vibrational bands (wavenumbers in cm-1) of the protonated carboxylic groups revealed by in situ H/D-exchange measurements.
| WT
|
E194D
|
E204D
|
|||||
|---|---|---|---|---|---|---|---|
| Intermediate | Residue | H2O | D2O | H2O | D2O | H2O | D2O |
| BR | D96 | 1,742 | 1,731 | 1,742 | 1,731 | 1,742 | 1,731 |
| D115 | 1,736 | 1,725 | 1,736 | 1,725 | 1,736 | 1,725 | |
| E/D204 | Deprotonated | 1,712 | 1,703 | 1,711 | 1,703 | ||
| M | D96 | 1,741 | 1,730 | 1,741 | 1,730 | 1,741 | 1,730 |
| D115 | 1,737 | 1,726 | 1,737 | 1,726 | 1,737 | 1,726 | |
| D85 | 1,761 | 1,749 | 1,763 | 1,750 | 1,761 | 1,749 | |
| E/D194 | Deprotonated | 1,718 | 1,706 | Deprotonated | |||
The wavenumbers in italics are results obtained from D96N or D115N mutants. The measurements of the D96 and D115 bands in M are not shown in this article.